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| Registros recuperados : 61 | |
| 21. |  | ROMÃO-DUMARESQ, A. S.; DOURADO, M. N.; FAVARO, L. C. de L.; MENDES, R.; FERREIRA, A.; ARAÚJO, W. L. Diversity of cultivated fungi associated with conventional and transgenic sugarcane and the interaction between endophytic trichoderma virens and host plant. Plos One, v. 11, n. 7, p. e0158974, 2016.| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Meio Ambiente. |
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| 22. | | TORRES, A. R.; ARAÚJO, W. L.; CURSINO, L.; HUNGRIA, M.; PLOTEGHER, F.; MOSTASSO, F. L.; AZEVEDO, J. L. Diversity of endophytic enterobacteria associated with different host plants. The Journal of Microbiology, Korea, v. 46, n. 4, p. 373-379, Ago. 2008.| Biblioteca(s): Embrapa Soja. |
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| 24. | | FERREIRA, A. J.; ROJAS, J. D.; FAVARO, L. C. de L.; BRAGA, R. M.; ARAUJO, W. L. Desenvolvimento de ferramentas na análise de genes de Epicoccum nigrum. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal, RN. Abstracts... São Paulo: Sociedade Brasileira de Microbiologia, 2013.| Biblioteca(s): Embrapa Agroenergia. |
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| 26. | | FERREIRA, A. J.; OLIVEIRA, L. S.; ALVES, J. M. P.; THON, M.; DURHAM, A. M.; FAVARO, L. C. de L.; GRUBER, A.; ARAÚJO, W. L. Genome analysis of E. nigrum and other filamentous fungi reveals molecular mechanisms related to endophytic/pathogenic lifestyles. In: X-MEETING; INTERNATIONAL CONFERENCE OF the AB3C, 2016, Belo Horizonte, MG. [Anais ... ]. Ribeirão Preto: AB3C, 2016. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 27. | | FAVARO, L. C. de L.; SEBASTINES, F. L. S.; LACAVA, P. T.; RODRIGUES, M. B. C.; ARAÚJO, W. L.; AZEVEDO, J. L.; KLEINER, A. A. P. Genetic transformation of Diaporthe phaseolorum, an endophytic fungus found in mangrove forests, mediated by Agrobacterium tumefaciens. Current Genetics, v. 58, p. 21?33, 2012. 21-33| Biblioteca(s): Embrapa Agroenergia. |
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| 28. | | SEBASTIANES, F. L. S.; LACAVA, P. T.; FAVARO, L. C. de L.; RODRIGUES, M. B. C.; ARAÚJO, W. L.; AZEVEDO, J. L.; PIZZIRANI-KLEINER, A. A. Genetic transformation of Diaporthe phaseolorum, an endophytic fungus found in mangrove forests, mediated by Agrobacterium tumefaciens. Current Genetics, Dec. 2011. Não paginado. DOI 10.1007/s00294-011-0362-2.| Biblioteca(s): Embrapa Agroenergia. |
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| 29. | | LACAVA, P. T.; DIAS, A.; SANTOS, V. C.; FERREIRA, A.; QUECINE, M. C.; ARAÚJO, W. L.; MELO, I. S. de; AZEVEDO, J. L. Analysis and characterization of the bacteria community associated with tropical mangrove forest. In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 13., 2007, Sorrento. Book of abstracts... Sorrento, 2007. p.238.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 30. | | MARTINS, P. C.; CAMPOS, M. M.; JAYME, C. G.; MACHADO, F. S.; TOMICH, T. R.; PEREIRA, L. G. R.; SILVA, R. B. e; ARAÚJO, W. L. de P. Levantamento das técnicas zootécnicas utilizadas na criação de bezerras leiteiras no município de Rio Pomba, MG. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 15., 2015, Juiz de Fora, MG. Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. (Embrapa Gado de Leite. Documentos, 176.). 1 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 31. | | SIQUEIRA, J. A.; BARROS, J. A. S.; DAL-BIANCO, M.; MARTINS, S. C. V.; MAGALHAES, P. C.; DAMATTA, F. M.; ARAÚJO, W. L.; RIBEIRO, C. Metabolic and physiological adjustments of maize leaves in response to aluminum stress. Theoretical and Experimental Plant Physiology, v. 32, n. 2, p. 133-145, 2020.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 32. | | DIAS, A. C. F.; ANDREOTE, F. D.; DINI-ANDREOTE, F.; LACAVA, P. T.; SÁ, A. L. B.; MELO, I. S. de; AZEVEDO, J. L.; ARAUJO, W. L. Diversity and biotechnological potential of culturable bacteria from Brazilian mangrove sediment. World Journal of Microbiology and Biotechnology, v. 25, n. 7, p. 1305-1311, 2009.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 33. | | LACAVA, P. T.; SILVA-STENICO, M. E.; ARAÚJO, W. L.; SIMIONATO, A. V. C.; CARRILHO, E.; TSAI, S. M.; AZEVEDO, J. L. Detection of siderophores in endophytic bacteria Methylobacterium spp. associated with Xylella fastidiosa subsp. pauca. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 4, p. 521-528, abr. 2008 Título em português: Detecção de sideróforos nas bactérias endofíticas Methylobacterium spp. associadas com Xylella fastidiosa subsp. pauca.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 34. | | SOUSA, G. P.; MENDES, T. D.; FRANCO, P. F.; ARAÚJO, W. L.; RODRIGUES, D. de S.; SALUM, T. F. C.; FAVARO, L. C. de L. Fungos endofíticos de cana-de-açúcar e seu potencial para produção de enzimas lignocelulolíticas. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos, SP. [Anais ...]. Brasília, DF: SBRG, 2014. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 35. | | GOMES, T. C.; CONRADO, R.; OLIVEIRA, R. C. de; SELARI, P. J. R. G.; MELO, I. S. de; ARAÚJO, W. L.; MARIA, D. A.; SOUZA, A. O. de. Effect of Monocerin, a fungal secondary metabolite, on endothelial cells. Toxins v. 15, n. 5, article 344, 2023.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 36. | | Quecine, M. C.; Araújo, W. L.; Rosseto, P. B.; FERREIRA, A.; TSUI, S.; Lacava, P. T.; Mondin, M.; Azevedo, J. L.; Pizzirani-Kleiner, A. A. Sugarcane growth promotion by the endophytic bacterium Pantoea agglomerans 33.1. Applied and Environmental Microbiology, v. 78, n. 21, p. 7511-7518, 2012.| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 37. | | CHAPPER, M.; LOUREIRO, M. E.; MOSQUIM, P. R.; ARAÚJO, W. L.; PEREIRA, A. da S.; FINGER, F. L.; SIMÕES, A. N. Mudanças metabólicas após recondicionamento a 15 C de tubérculos de batata armazenados a baixa temperatura. Horticultura Brasileira, Brasília, DF, v. 22, n. 4, p. 700-705, out./dez. 2006.| Biblioteca(s): Embrapa Hortaliças. |
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| 38. | | BATISTA, K. D.; ARAÚJO, W. L.; ANTUNES, W. C.; CAVATTE, P. C.; MORAES, G. A. B. K.; MARTINS, S. C. V.; DAMATTA, F. M. Photosynthetic limitations in coffee plants are chiefly governed by diffusive factors. Trees, v. 26, p. 459-468, 2012.| Biblioteca(s): Embrapa Roraima. |
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| 39. | | SOUSA, G. P.; JARAMILLO, P. M. D.; MENDES, T. D.; SALUM, T. F. C.; ARAÚJO, W. L.; FAVARO, L. C. de L. Prospecção de fungos endofíticos de cana-de-açúcar para produção de enzimas lignocelulolítcas. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA A AMÉRICA LATINA E CARIBE, 10., 2015, Bento Gonçalves. Recursos genéticos no século 21: de Vavilov a Svalbard. Anais... [s.l.]: Sociedade Brasileira de Recursos Genéticos, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 40. | | FAVARO, L. C. de L.; MELO, F. L.; ARAÚJO, F. D. S.; MARSAIOLI, A. J.; SILVA, M. A.; ROJAS, R. D.; ARAÚJO, W. L. Rational bioprospecting and identi cation of genes from sugarcane endophytic fungi Epicoccum nigrum and Epicoccum sp. involved in the biosynthesis of bioactive metabolites. In: REUNIÃO DE GENÉTICA DE MICRORGANISMOS - REGEM, 28., 2012, Foz do Iguaçu, PR. [Anais...]. Ribeirão Preto: Sociedade Brasileira de Genética, 2012. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 61 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
23/10/2017 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FAJARDO, T. V. M.; SILVA, F. N.; EIRAS, M.; NICKEL, O. |
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Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; Fábio N. Silva, Universidade do Estado de Santa Catarina, Lages, SC 88520-000, Brazil; Marcelo Eiras, Instituto Biológico de São Paulo, São Paulo, SP 04014-002, Brazil; OSMAR NICKEL, CNPUV. |
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Título: |
High-throughput sequencing applied for the identification of viruses infecting grapevines in Brazil and genetic variability analysis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 52, p. 250-260, 2017. |
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DOI: |
10.1007/s40858-017-0142-8 |
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Idioma: |
Português |
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Conteúdo: |
The application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, and that recombination events occurred in the majority of the viral species analyzed. The coat protein genes, generally, had lower genetic variability than the replicase and movement protein genes. Keywords Vitis . Diagnosis . HTS . Next-generation sequencing . NGS . Variability MenosThe application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, an... Mostrar Tudo |
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Palavras-Chave: |
Diagnosis; HTS; Next-generation sequencing; NGS. |
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Thesaurus NAL: |
variability; Vitis. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/197010/1/Fajardo2017-Article-High-throughputSequencingAppli.pdf
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Marc: |
LEADER 02498naa a2200241 a 4500
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008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s40858-017-0142-8$2DOI
100 1 $aFAJARDO, T. V. M.
245 $aHigh-throughput sequencing applied for the identification of viruses infecting grapevines in Brazil and genetic variability analysis.$h[electronic resource]
260 $c2017
520 $aThe application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes. Grapevine virus infections are usually mixed and the HTS assays were suitable to identify ten viruses already reported that traditionally infect grapevines in Brazil, one that has been recently identified (Grapevine Syrah virus 1) and others (Grapevine Cabernet Sauvignon reovirus, Grapevine Red Globe virus and Grapevine vein clearing virus) not previously reported in this country. Nucleotide identities among Brazilian isolates identified by HTS and homologous grapevine virus sequences in GenBank were high, ranging from 77% to 99%. Genetic variability analysis of viral sequences obtained by HTS and sequences available in GenBank indicated that the coding regions in the different viral species are under purifying selection, and that recombination events occurred in the majority of the viral species analyzed. The coat protein genes, generally, had lower genetic variability than the replicase and movement protein genes. Keywords Vitis . Diagnosis . HTS . Next-generation sequencing . NGS . Variability
650 $avariability
650 $aVitis
653 $aDiagnosis
653 $aHTS
653 $aNext-generation sequencing
653 $aNGS
700 1 $aSILVA, F. N.
700 1 $aEIRAS, M.
700 1 $aNICKEL, O.
773 $tTropical Plant Pathology$gv. 52, p. 250-260, 2017.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| 1. | | 0900875, RELATORIO TECNICO ANUAL. EMBRAPA. CNPSA, EMBRAPA-CNPSA, Concordia-SC
Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Acre; Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Pantanal. | |
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